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Product Info Summary
| SKU: | A13391 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZSCAN4 Antibody
SKU/Catalog Number
A13391
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZSCAN4 Antibody (Catalog # A13391). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZSCAN4 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZSCAN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13391)
Host
Rabbit
Contents
ZSCAN4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ZSCAN4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4. The immunogen is located within amino acids 370 - 420 of ZSCAN4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of JAKMIP2 are known to exist; this antibody will detect all four isoforms. JAKMIP2 antibody is predicted to not cross-react with JAKMIP1.
Reactive Species
A13391 is reactive to ZSCAN4 in Human, Mouse, Rat
Applications
A13391 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.957kDa
Background of ZSCAN4
Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZSCAN4 antibody can be used for detection of ZSCAN4 by Western blot. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZSCAN4 in mouse lung tissue lysate with ZSCAN4 antibody in (A) the absence and (B) presence of blocking peptide.
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Immunohistochemistry of ZSCAN4 in rat lung tissue with ZSCAN4 antibody at 5 μg/mL.
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Immunofluorescence of ZSCAN4 in Rat Lung tissue with ZSCAN4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZSCAN4 (Source: Uniprot.org, NCBI)
Gene Name
ZSCAN4
Full Name
Zinc finger and SCAN domain-containing protein 4
Weight
48.957kDa
Alternative Names
FLJ35105; MGC126787; zinc finger and SCAN domain containing 4; Zinc finger protein 494MGC126789; ZNF494zinc finger and SCAN domain-containing protein 4 ZSCAN4 ZNF494 zinc finger and SCAN domain containing 4 zinc finger and SCAN domain-containing protein 4|zinc finger protein 494
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZSCAN4, check out the ZSCAN4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZSCAN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZSCAN4 Antibody (A13391)
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No publications found for A13391
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Customer Reviews
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